Syllabus Section 3 - Microscopy

3.1

The candidate will be able to give:

3.1.1

a simple account of the light microscope (both high power compound and binocular dissecting), its components and its use.

3.1.2

a definition and show an understanding of the following terms used in light microscopy: Numerical Aperture; Magnification; Useless Magnification; Critical illumination; Koehler Illumination; Condenser Aperture; Oil Immersion.

3.1.3

a simple account of the electron microscope and its uses.

 

3.2

The candidate will be able to give an in-depth account of:

3.2.1

the preparation of objects for examination using both temporary and permanent mounts.

3.2.2

simple routine staining methods used for animal and plant material.

3.2.3

the use of the microscope in beekeeping for disease diagnosis and pollen identification.

3.2.4

the techniques used to dissect the honey bee.


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